Device

Part:BBa_K4813004:Design

Designed by: YuHin Lau   Group: iGEM23_HongKong-JSS   (2023-10-03)


pFrmR-tdTomato formaldehyde sensing chromoprotein reporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1298
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1298
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1298
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1298
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 235


Design Notes

NIL


Source

The original tdTomato sequence was obtained from NCBI. Then, the sequence was codon optimized by IDT and gBlock was synthesized from IDT.

References